Web Application for FLT3-ITD Nomenclature

If you want to use it for clinical reporting, make sure you complete a clinical validation.
FLT3 RefSeq cDNA: NM_004119.2. The reference chromsome assembly is hg19, GRCh37.

Paste your assembled FLT3-ITD sequencing result into the box below (only simple text with A T C G N allowed);
(The box is pre-populated with an example for you to test, clear the example before pasting your sequence in):

To cite this web app:
Ding, Y., Smith, G., Deeb, K., Schneider, T., Campbell A., Zhang, L. Revealing molecular architecture of FLT3 internal tandem duplication: Development and clinical validation of a web-based application to generate accurate nomenclature. Int J Lab Hematol. 2022 Oct;44(5):918-927. doi: 10.1111/ijlh.13930 (PMID: 35795913)

Potential problems of current version (v4.0 updated on 02/28/2023):
1. If there are double duplications/insertions (triplication, very rare), this web app will not create correct nomenclature. There will be an alert in "Additional information".
2. If there is mutation/insertion at the 3'-end of the ITD, position search may fail, and the inserted NT or AA may not be recognized correctly. This is reported in the "Additional information" section.
3. Due to our limited understanding of RNA splicing, processing of intronic sequence might not be entirely correct (also reported in the "Additional information" section).
4. Only works when the duplicated segment is >18 bp (even the whole ITD itself may be longer). Note: Most routine bioinformatics pipelines should be able to create an accurate nomenclature when ITD is <24 bp; you don't need to use this webapp.

Your sequence submitted for nomenclature is not stored in this web app.

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